Figure 5
From: MicroRNA-124 promotes hepatic triglyceride accumulation through targeting tribbles homolog 3
miR-124 regulates TRB3 expression.
(A) Western blot analysis of TRB3 and phospho-Akt in the liver of mice infected with Ad-GFP or Ad-miR-124. (B) Western blot analysis of TRB3 and phospho-Akt in HepG2 cells treated with miR-124 mimics (100 nM) or vehicle control for 48 hours. (C) Western blot analysis of TRB3 and phospho-Akt in Hepa1-6 cells treated with Ad-GFP or Ad-miR-124 (100MOI) for 72 hours. (D,E) Relative luciferase activity of the firefly reporter containing the 3′UTR of TRB3 was detected in HEK293T cells (D) and HepG2 cells (E) co-transfected with pri-miR-124. (F) Sequence alignment of the 3′UTRs of TRB3 and miR-124. (G) Relative luciferase activity of reporters containing either the wild-type or mutated 3′UTRs of TRB3 gene (Mut1, Mut2, Mut3 and Mut1 + 2 + 3) were also analyzed in HEK293T cells. *P < 0.05, ***P < 0.001.
