Figure 8
IYIY-I2-BODIPY combined with PDT induces stronger adaptive immunity.
4T1 tumor bearing mice were treated with respective compounds via tail vain. Mice were kept in dark for 1 h, anaesthetized and irradiated with 100 J/cm2 of light at a fluence rate of 160 mW/cm2. Mice were then sacrificed at 2 h and 24 h post-PDT. Cytokines and immune cells quantification were examined based on the methods explained in M&M. Graph of fold changes in (a) blood cytokines and (b) immune cells populations in TM of the light (Supplementary Figure 3) versus dark (Figs 2, 3 and 4) for 2 h and 24 h in IYIY-I2-BODIPY treated group. Fold changes for each sample in IYIY-I2-BODIPY treated group was obtained by dividing the percentage in the light over the percentage in the dark. *p < 0.05, ***p < 0.001 vs IYIY-I2-BODIPY in the dark using student’s t-test.
