Figure 4: BgSvp-B controls JH production in B. germanica adult females. | Scientific Reports

Figure 4: BgSvp-B controls JH production in B. germanica adult females.

From: Juvenile hormone biosynthesis in adult Blattella germanica requires nuclear receptors Seven-up and FTZ-F1

Figure 4

(A) Expression patterns of BgSvp-B, relative to BgActin5C levels, in the CA of B. germanica females during the last nymphal instar and the adult stage, measured by qRT-PCR. (B–D) Newly emerged sixth instar female nymphs were injected with 3 μg of dsBgSvp-A (BgSvp-Ai), dsBgSvp-B (BgSvp-Bi) or with dsMock (Control). (B) Rates of JH synthesis by CA incubated in vitro from 5-day-old Control, BgSvp-Ai and BgSvp-Bi adult females. (C) Expression levels of BgVg, relative to BgActin5C levels, in the fat body of 5-day-old BgSvp-Bi adult females treated with acetone or methoprene, compared to acetone-treated Control adults, measured by qRT-PCR. (D) Basal oocyte length of 5-day-old BgSvp-Bi adult females treated with acetone or methoprene, compared to acetone-treated Control adults. Error bars represent SEM (n = 3–10). Asterisks in (B) indicate differences statistically significant with respect to Controls (***p ≤ 0.0001; Student’s t test). Different letters in (C and D) represent groups with significant differences according to ANOVA test (Tukey, p ≤ 0.005).

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