This Article contains errors in the Results section under the subheading ‘Development of a pH-stable GFP variant for use as biomarker’,
“To create a monomeric GFP variant, we created a construct comprised of two sfGFP-N149Y/Q204H separated by a flexible 25 amino acid linker, termed pH-stable tandem dimer GFP (pH-tdGFP).”
should read:
“To create a monomeric GFP variant, we created a construct comprised of two sfGFP-N149Y/Q204H separated by a flexible 22 amino acid linker, termed pH-stable tandem dimer GFP (pH-tdGFP).”
Additionally, in the Materials and Methods section under the subheading ‘Purification of GFP variants’,
“pH-tdGFP is a tandem fusion of two yeast codon optimized sfGFP-N149Y/Q204H separated by the amino acid linker ITLGGTGSGSGDEVDGMVSKGEEVIK.”
should read:
“pH-tdGFP is a tandem fusion of two yeast codon optimized sfGFP-N149Y/Q204H separated by the amino acid linker HGTGSTGSGSSGTASSEDNNMA.”
Additional information
The online version of the original article can be found at https://doi.org/10.1038/srep28166
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Roberts, T., Rudolf, F., Meyer, A. et al. Correction: Corrigendum: Identification and Characterisation of a pH-stable GFP. Sci Rep 8, 46976 (2018). https://doi.org/10.1038/srep46976
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DOI: https://doi.org/10.1038/srep46976
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