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DNA origami–based 'nanocalipers' present ligands at well-defined positions to enable probing of the spatial regulation of membrane receptor function. Artwork by Björn Högberg. Article p841
Managing, sharing and controlling the quality of plasmids can be simple. But many techniques to disseminate them do not scale, which leads nonprofits and companies to explore new options.
Barcoded semisynthetic nucleosomes combined with massively parallel sequencing provide an innovative new platform for analyzing the histone-recognition and histone-modifying activities of chromatin-associated proteins.
This Perspective discusses the power of large mutational scans for the study of protein properties, the analytical challenges posed by the resulting data sets and the potential of this approach to further our understanding of human genetic variation.
12 microRNA expression profiling platforms are compared for their reproducibility, sensitivity, accuracy and specificity, and the strengths and weaknesses of each platform are discussed.
This paper describes immunocompromised rag2 mutant zebrafish, which allow efficient and robust cell transplantations in adult zebrafish, thereby facilitating stem cell, cancer and regeneration research.
A combination of a sensitive blue light–gated channelrhodopsin actuator and red-shifted Arch-based voltage sensors allows all-optical electrophysiology without cross-talk in cultured neurons or brain slices.
A synthetic library of nucleosomes, each with a DNA-barcode characteristic for a defined post-translational modification (PTM), is used to probe PTM-based recruitment and modulation of histone mark readers and writers.
Ligand nanocalipers made using DNA nanotechnology are used to display ephrin-A5 at well-defined spatial intervals to study how nanoscale ligand spacing affects EphA2 receptor activation in breast cancer cells.
This paper demonstrates that micropatterned human embryonic stem cell colonies can acquire spatial patterns reminiscent of those in the embryo and proposes their use to study early developmental processes in the human system.
A simple, robust, chemically defined method for generating cardiomyocytes from human pluripotent stem cells is described. It should enable the identification of conditions for maturation of these cells.
A tetramethylrhodamine-labeled dimer of the cell-penetrating peptide TAT, named dfTAT, efficiently delivers proteins into live cells by facilitating endosomal escape.
The results of genome-wide association studies are combined with quantitative interaction proteomics to narrow down the list of putative causal disease genes and filter modest association signals.