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Showing 1–8 of 8 results
Advanced filters: Author: "Guillermo Montoya" Clear advanced filters

  • The Class 2 family of CRISPR nucleases named Cas12j, which shares only low sequence identity with other CRISPR nucleases was recently identified in the biggiephage clade of phages. Here, the authors present the cryo-EM structure of a functional Cas12j3−crRNA complex in the post-catalytic state and discuss Cas12j3 PAM recognition, hybrid stabilisation and the activation mechanism.

    • Arturo Carabias
    • Anders Fuglsang
    • Guillermo Montoya
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-12

  • Type III CRISPR-Cas RNases from the Csm and Csx families are activated by cyclic oligoadenylates (cOA). Here the authors present the cOA bound Sulfolobus islandicus Csx1 structure, which forms a hexamer and reveal an allosteric mechanism for the activation of Csx1 RNase.

    • Rafael Molina
    • Stefano Stella
    • Guillermo Montoya
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-14

  • chTOG, a microtubule polymerase, interacts with TACC3 during mitosis to regulate spindle formation. By studying their Xenopus homologues, Mortuza et al. discover that one TACC3 recruits two chTOG molecules to the spindle, increasing its local concentration and promoting microtubule elongation.

    • Gulnahar B. Mortuza
    • Tommaso Cavazza
    • Guillermo Montoya
    ResearchOpen Access
    Nature Communications
    Volume: 5, P: 1-12

  • The Tousled-like kinase (TLKs) family belongs to a distinct branch of Ser/Thr kinases that exhibit the highest levels of activity during DNA replication. Here the authors present the crystal structure of the kinase domain from human TLK2 and propose an activation model for TLK2 based on biochemical and phosphoproteomics experiments.

    • Gulnahar B. Mortuza
    • Dario Hermida
    • Guillermo Montoya
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-17

  • Different catalytic steps of endonuclease I-DmoI are captured crystallographically to allow direct observation of the generation of a DNA double-strand break. A third metal ion enters the active site and has a key role in hydrolysis.

    • Rafael Molina
    • Stefano Stella
    • Guillermo Montoya
    Research
    Nature Structural & Molecular Biology
    Volume: 22, P: 65-72

  • This review highlights recent mechanistic insights into the CRISPR class 2 type V enzymes Cpf1 and C2c1, which are crucial for improving these genome engineering tools and expanding the genomic editing space.

    • Stefano Stella
    • Pablo Alcón
    • Guillermo Montoya
    Reviews
    Nature Structural & Molecular Biology
    Volume: 24, P: 882-892