In this Review, Claassenset al. discuss recent advances in microbial autotrophic production platforms, in particular the genetic engineering of autotrophic systems in autotrophic microorganisms, the transplantation of partial or complete CO2fixation pathways and autotrophic energy-harvesting systems into heterotrophs, and the large potential of hybrid systems as autotrophic production platforms. They also explore strategies for the design, quantitative analysis and modular experimental implementation of autotrophic cell factories.
- Nico J. Claassens
- Diana Z. Sousa
- John van der Oost