Abstract
IN a recent paper1, it was shown by means of paper chromatography that in bleeding sap of pea plants 5½ weeks old, inoculated with effective strains of Rhizobium, some amino-acids and amides occurred. The chief of these compounds proved to be aspartic acid, asparagine, glutamine and a substance giving a colour with ninhydrin, of which the R F values on nearly all chromatograms corresponded with those of threonine. Only when using phenol/water as solvent was a somewhat higher R F value found. The other solvents used were : collidine/water; butanol/acetic acid/water ; propanol/water2.
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BAKHUIS, J. Homoserine in Bleeding Sap of Pea Plants. Nature 180, 713 (1957). https://doi.org/10.1038/180713a0
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DOI: https://doi.org/10.1038/180713a0
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