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Biological Activity of a Brownish-Yellow Pigment produced from Vitamin B12 by Aerobacter aerogenes

Abstract

IT has previously1 been shown that two brownish-yellow pigments could be isolated from the incubation mixture when a strain of Aerobacter aerogenes is grown in the presence of vitamin B12 in a glucose-salt medium. The pigment (pigment I) present in greatest quantity was shown to be derived from vitamin B12. It differed from vitamin B12 in its absorption spectrum and its electrophoretic and chromatographic properties. It was stable to air and light and was not as the cobamide coenzymes converted to aquocobalamin by the addition of potassium cyanide.

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References

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HELGELAND, K., JONSEN, J., LALAND, S. et al. Biological Activity of a Brownish-Yellow Pigment produced from Vitamin B12 by Aerobacter aerogenes. Nature 199, 604–605 (1963). https://doi.org/10.1038/199604a0

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