Abstract
DURING work on the irradiation of bakers' yeast, Saccharomyces cerevisiae, it was found that irradiation of the buffers, sodium cacodylate (sodium dimethylarsinate), (CH3)2 As O O Na, and of tris (hydroxymethyl) aminomethane (=tris= 2-amino-2-hydroxymethyl-1, 3-propanediol), yielded substances giving positive Fiske–SubbaRow1 and ninhydrin2 colorimetric tests, respectively. Further investigations have been carried out to assess the value of these systems in radiation dosimetry.
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References
Fiske, C. H., and SubbaRow, Y., J. Biol. Chem., 63, 375 (1925).
Moore, S., and Stein, W. H., J. Biol. Chem., 176, 367 (1948).
Samples irradiated in ‘High Temp’ plastic culture tubes supplied by Falcon Plastics Co., Division of Becton, Dickinson and Co.
To receive 105 rads, the conveyor travelled at about 3.5 ft. /min through the 4.72 ft. of the source.
Hart, E. J., and Walsh, P. D., Radiat. Res., 1, 342 (1954).
β-Irradiation with the 2.0-MeV Van de Graaff Electron Accelerator at Electronized Chemicals Corp., Burlington, Mass.
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LEVINSON, H., GARBER, E. Radiation Dosimetry with Sodium Cacodylate and Tris. Nature 207, 751–752 (1965). https://doi.org/10.1038/207751a0
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DOI: https://doi.org/10.1038/207751a0
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