Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Sequence and structure of D-glyceraldehyde 3-phosphate dehydrogenase from Bacillus stearothermophilus

Nature volume 266, pages 328–333 (1977)Cite this article

Abstract

The glyceraldehyde 3-phosphate dehydogenase holoenzyme of Bacillus stearothermophilus possesses precise 222 symmetry: in this respect it differs from the reported structure of the lobster muscle enzyme. Pairs of active sites are linked through a flexible polypeptide loop which probably mediates the structural changes giving rise to cooperative effects. Three additional salt bridges made by each subunit to others would make a major contribution to thermostability of the tetramer.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Harris, J. I. & Waters, M. in The Enzymes XIII, 3rd edn (ed. P. D. Boyer) 1–50 (Academic, New York, 1976).

    Google Scholar 

  2. Harris, J. I. & Perham, R. N. J. molec. Biol. 13, 876–88 (1965).

    Article  CAS  Google Scholar 

  3. Conway, A. & Koshland, D. E., Jr. Biochemistry 7, 4011–4022 (1968).

    Article  CAS  Google Scholar 

  4. de Vijlider, J. J., Hilvers, A. G., van Lis, J. M. J. & Slater, E. C. Biochem. biophys. Acta 191, 221–228 (1969).

    Google Scholar 

  5. Seydoux, F., Bernhard, S., Pfenninger, O., Payne, M. & Malhotra, O. P. Biochemistry 12, 4290–4300 (1973).

    Article  CAS  Google Scholar 

  6. Cook, R. A. & Koshland, D. E., Jr. Biochemistry 9, 3337–3342 (1970).

    Article  CAS  Google Scholar 

  7. Kirschner, K. J. molec. Biol. 58, 51–68 (1971).

    Article  CAS  Google Scholar 

  8. Monod, J., Wyman, J. & Changeux, J. P. J. molec. Biol. 12, 88–118 (1965).

    Article  CAS  Google Scholar 

  9. Koshland, D. E., Jr., Némethy, G. & Filmer, D. Biochemistry 5, 365–385 (1966).

    Article  CAS  Google Scholar 

  10. Kirtley, M. E. & Koshland, D. E., Jr. J. biol. Chem. 242, 4192–4205 (1967).

    CAS  PubMed  Google Scholar 

  11. Bernhard, S. A. & MacQuarrie, R. A. J. molec. Biol. 74, 73–78 (1973).

    Article  CAS  Google Scholar 

  12. Moras, D. et al. J. biol. Chem. 250, 9137–9162 (1975).

    CAS  PubMed  Google Scholar 

  13. Thierry, J. C., Biesecker, G., Wonacott, A. J., Butler, R. & Sweet, R. M. (in preparation).

  14. Singleton, R., Kimmel, J. R. & Amelunxen, R. E. J. biol. Chem. 244, 1623–1630 (1969).

    CAS  PubMed  Google Scholar 

  15. Suzuki, K. & Harris, J. I. FEBS Lett. 13, 217–220 (1971).

    Article  CAS  Google Scholar 

  16. Allen, G. & Harris, J. I. Biochem. J. 151, 747–749 (1975).

    Article  CAS  Google Scholar 

  17. Buehner, M., Ford, G. C., Moras, D., Olsen, K. W. & Rossmann, M. G. Proc. natn. Acad. Sci. U.S.A. 70, 3052–3054 (1973).

    Article  ADS  CAS  Google Scholar 

  18. Buehner, M., Ford, G. C., Moras, D., Olsen, K. W. & Rossmann, M. G. J. molec. Biol. 90, 25–40 (1974).

    Article  CAS  Google Scholar 

  19. Hocking, J. D. & Harris, J. I. FEBS Lett. 34, 280–284 (1973).

    Article  CAS  Google Scholar 

  20. Walker, J. E., Bridgen, J. & Harris, J. I. (in preparation).

  21. Arndt, U. W., Champness, J. N., Phizackerley, R. P. & Wonacott, A. J. J. appl. Crystallogr. 6, 457–463 (1973).

    Article  CAS  Google Scholar 

  22. Bricogne, G. Acta crystallogr. A 30, 395–405 (1974).

    Article  CAS  Google Scholar 

  23. Bricogne, G. Acta crystallogr. A 32, 832–847 (1976).

    Article  Google Scholar 

  24. Davidson, B. E., Sajgò, M., Noller, H. F. & Harris, J. I. Nature 216, 1181–1185 (1967).

    Article  ADS  CAS  Google Scholar 

  25. Harris, J. I., Meriwether, B. P. & Park, J. H. Nature 198, 154–157 (1963).

    Article  ADS  CAS  Google Scholar 

  26. Park, J. H., Agnello, C. F., Mathew, E. J. biol. Chem. 241, 769–771 (1966).

    CAS  PubMed  Google Scholar 

  27. Harris, J. I. & Polgár, L. J. molec. Biol. 14, 630–633 (1965).

    Article  CAS  Google Scholar 

  28. Hill, E., Tsernoglou, D., Webb, L. & Banaszak, L. J. J. molec. Biol. 72, 577–589 (1972).

    Article  CAS  Google Scholar 

  29. Brändén, C. I. et al. Proc. natn. Acad. Sci. U.S.A. 70, 2439–2442 (1973).

    Article  ADS  Google Scholar 

  30. Adams, M. J., Ford, G. C., Liljas, A. & Rossmann, M. G. Biochem. biophys. Res. Commun. 53, 46–51 (1973).

    Article  CAS  Google Scholar 

  31. Rossmann, M. G. et al. J. molec. Biol. 76, 533–537 (1973).

    Article  CAS  Google Scholar 

  32. Olsen, K. W., Moras, D., Rossmann, M. G. & Harris, J. I. J. biol. Chem. 250, 9313–9321 (1975).

    CAS  PubMed  Google Scholar 

  33. Trentham, D. R. Biochem. J. 122, 59–69 (1970).

    Article  Google Scholar 

  34. Harrigan, P. J. & Trentham, D. R. Biochem. J. 143, 353–363 (1974).

    Article  CAS  Google Scholar 

  35. Byers, L. O. & Koshland, D. E. Biochemistry 14, 3661–3669 (1975).

    Article  CAS  Google Scholar 

  36. Racker, E. & Krimsky, I. J. biol. Chem. 198, 731–743 (1952).

    CAS  PubMed  Google Scholar 

  37. Kosower, E. M. J. Am. chem. Soc. 78, 3497–3501 (1956).

    Article  CAS  Google Scholar 

  38. Kosower, E. M. & Klinedinst, D. E., Jr. J. Am. chem. Soc. 78, 3493–3497 (1965).

    Article  Google Scholar 

  39. Amelunxen, R. E. Biochim. biophys. Acta. 139, 24–32 (1967).

    Article  CAS  Google Scholar 

  40. Amelunxen, R. E., Noelkin, M. & Singleton, R., Jr. Archs Biochem. Biophys. 141, 447–455 (1970).

    Article  CAS  Google Scholar 

  41. Hocking, J. D. & Harris, J. I. in Enzymes and Proteins from Thermophilic microorganisms. (ed. Zuber, H.) 121–134 (Birkhausen Verlag, Basle & Stuttgart, 1976).

    Book  Google Scholar 

  42. Hocking, J. D. thesis, Univ. Cambridge (1974).

  43. Perutz, M. F. & Raidt, H. Nature 255, 256–259 (1975).

    Article  ADS  CAS  Google Scholar 

  44. Matthews, B. W., Weaver, L. H. & Kester, W. R. J. biol. Chem. 249, 8030–8044 (1974).

    CAS  Google Scholar 

  45. Bricogne, G., Thierry, J. C. & Wonacott, A. J. (in preparation).

  46. Fletterick, R. J. & Steitz, T. A. Acta crystallogr. A 32, 125–132 (1976).

    Article  Google Scholar 

  47. Biesecker, A., Thierry, J. C. & Wonacott, A. J. (in preparation).

  48. Fuller Noel, J. K. & Schumaker, V. W. J. molec. Biol. 68, 523–532 (1972).

    Article  CAS  Google Scholar 

  49. Sloan, D. L. & Velick, S. F. J. biol. Chem. 248, 5419–5423 (1973).

    CAS  PubMed  Google Scholar 

  50. Smith, G. D. & Schachman, H. K. Biochemistry 12, 3789–3801 (1973).

    Article  CAS  Google Scholar 

  51. Durchschlag, H., Puchwein, G., Kratky, O., Schuster, I. & Eur. J. Biochem. 19, 9–22 (1971).

    Article  CAS  Google Scholar 

  52. Simon, I., Eur. J. Biochem. 30, 184–189 (1972).

    Article  CAS  Google Scholar 

  53. Bornstein, P. Biochemistry 9, 2408–2420 (1970).

    Article  CAS  Google Scholar 

  54. Ommer, G. S., Fontana, A. & Anfinsen, C. B. J. biol. Chem. 245, 1895–1902 (1970).

    Google Scholar 

  55. Hartley, B. S. Biochem. J. 119, 805–822 (1970).

    Article  CAS  Google Scholar 

  56. Houmard, J. & Drapeau, G. R. Proc. natn. Acad. Sci. U.S.A. 69, 3506–3509 (1972).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Author notes

  1. G. Biesecker

    Present address: Scripps Clinic & Research Foundation, 476 Prospect Street, La Jolla, California, 92037

  2. J. C. Thierry

    Present address: Institut de Chimie, 1 Rue Blaise Pascal, 67008, Strasbourg Cedex, France

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, UK

    G. Biesecker, J. Ieuan Harris, J. C. Thierry, J. E. Walker & A. J. Wonacott

Authors
  1. G. Biesecker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Ieuan Harris
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. C. Thierry
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. E. Walker
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. J. Wonacott
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Biesecker, G., Ieuan Harris, J., Thierry, J. et al. Sequence and structure of D-glyceraldehyde 3-phosphate dehydrogenase from Bacillus stearothermophilus. Nature 266, 328–333 (1977). https://doi.org/10.1038/266328a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/266328a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing