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Regulation of fast inactivation of cloned mammalian IK(A) channels by cysteine oxidation

Nature volume 352, pages 711–714 (1991)Cite this article

Abstract

MODULATION of neuronal excitability by regulation of K+ channels potentially plays a part in short-term memory1 but has not yet been studied at the molecular level. Regulation of K+ channels by protein phosphorylation2–5 and oxygen6 has been described for various tissues and cell types; regulation of fast-inactivating K+ channels mediating IK(A) currents has not yet been described. Functional expression of cloned mammalian K+ channels7–13 has provided a tool for studying their regulation at the molecular level. We report here that fast-inactivating K+ currents mediated by cloned K+ channel subunits derived from mammalian brain expressed in Xenopus oocytes are regulated by the reducing agent glutathione. This type of regulation may have a role in vivo to link metabolism to excitability and to regulate excitability in specific membrane areas of mammalian neurons.

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References

  1. Abrams, T. W. & Kandel, E. R. Trends Neurosci. 11, 128–136 (1988).

    Article  CAS  Google Scholar 

  2. Kaczmarek, L. K. Advances in Second Messenger and Phosphoprotein Research 22, 113–138 (1988).

    Google Scholar 

  3. Walsh, K. B. & Kass, R. S. Science 242, 67–69 (1989).

    Article  ADS  Google Scholar 

  4. Kume, H., Takai, A., Tokuno, H. & Tomita, T. Nature 341, 152–154 (1989).

    Article  ADS  CAS  Google Scholar 

  5. Strong, J. A. & Kaczmarek, L. K. J. Neurosci. 6, 814–822 (1986).

    Article  CAS  Google Scholar 

  6. Ganfornina, M. D. & Lopez-Barneo, J. Proc. natn. Acad. Sci. U.S.A. 88, 2927–2930 (1991).

    Article  ADS  CAS  Google Scholar 

  7. Stühmer, W. et al., FEBS Lett. 242, 199–206 (1988).

    Article  Google Scholar 

  8. Christie, M. J., Adelman, J. P., Douglass, J. & North, R. A. Science 244, 221–224 (1989).

    Article  ADS  CAS  Google Scholar 

  9. Stühmer, W. et al. EMBO J. 8, 3235–3244 (1989).

    Article  Google Scholar 

  10. Frech, G. C., Van Dongen, A. M., Schuster, G., Brown, A. M. & Joho, R. H. Nature 340, 642–645 (1989).

    Article  ADS  CAS  Google Scholar 

  11. McKinnon, D. J. biol. Chem. 264, 8230–8236 (1989).

    CAS  PubMed  Google Scholar 

  12. Grupe, A. et al. EMBO J. 9, 1749–1756 (1990).

    Article  CAS  Google Scholar 

  13. McCormack, R., Vega-Saenz, E. C. & Rudy, B. Proc. natn. Acad. Sci. U.S.A. 87, 5227–5231 (1990).

    Article  ADS  CAS  Google Scholar 

  14. Schröter, K. H. et al. FEBS Lett. 278, 211–216 (1991).

    Article  Google Scholar 

  15. Ruppersberg, J. P. et al. Nature 345, 535–537 (1990).

    Article  ADS  CAS  Google Scholar 

  16. Stocker, M. et al. Proc. natn. Acad. Sci. U.S.A. 87, 8903–8907 (1990).

    Article  ADS  CAS  Google Scholar 

  17. Kramer, R. H. Neuron 2, 335–341 (1990).

    Article  Google Scholar 

  18. Hoshi, T., Zagotta, W. N. & Aldrich, R. W. Science 250, 533–538 (1990).

    Article  ADS  CAS  Google Scholar 

  19. Zagotta, W. N., Hoshi, T. & Aldrich, R. W. Science 250, 568–570 (1990).

    Article  ADS  CAS  Google Scholar 

  20. Alkon, D. L., Lederhendler, I. & Shoukimas, J. J. Science 215, 693–695 (1982).

    Article  ADS  CAS  Google Scholar 

  21. Stocker, M. et al. Proc. R. Soc. Lond. B (in the press).

  22. Baumann, A. et al. EMBO J. 7, 2457–2463 (1988).

    Article  CAS  Google Scholar 

  23. Frank, R. & Gausepohl, H. Modern Methods in Protein Chemistry 3 (ed. Tschesche, H.) 42–60 (de Gruyter, Berlin, New York, 1988).

    Google Scholar 

  24. Castro, B., Domroy, J. R., Evin, G. & Selve, C. J. chem. Res. 182, 2118–2122 (1977).

    Google Scholar 

  25. Herlitze, S. & Koenen, M. Gene 91, 143–147 (1990).

    Article  CAS  Google Scholar 

Download references

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Author notes

  1. Martin Stacker and Olaf Pongs: Ruhr Universität Bochum, Lehrstuhl für Biochemie, D-4600 Bochum, Germany

  2. Stefan H. Heinemann: Max-Planck-lnstitut für Biophysikalische Chemie, Abteilung Membranbiophysik, D-3400 Göttingen, Germany

  3. Rainer Frank: Zentrum für Molekulare Biologie, D-6900 Heidelberg, Germany

Authors and Affiliations

  1. Max-Planck-lnstitut für medizinische Forschung, Abteilung Zellphysiologie, Jahnstrasse 29, D-6900, Heidelberg, Germany

    J. Peter Ruppersberg, Martin Stacker, Olaf Pongs, Stefan H. Heinemann, Rainer Frank & Michael Koenen

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  1. J. Peter Ruppersberg
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  4. Stefan H. Heinemann
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  6. Michael Koenen
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Ruppersberg, J., Stacker, M., Pongs, O. et al. Regulation of fast inactivation of cloned mammalian IK(A) channels by cysteine oxidation. Nature 352, 711–714 (1991). https://doi.org/10.1038/352711a0

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