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References
Borlaug, N.E. and Dowswell, C.R. 1988. World revolution in agriculture, pp. 5–14 in 1988 Book of the Year. Encyclopedia Brittanica, Chicago.
Vasil, I.K. 1988. The contributions and prospects of plant biotechnology—an assessment, pp. 15–19 in Plant Cell Biotechnology. M. S. S. Pais, F. Mavituna, and J. M. Novais (eds.), Springer-Verlag, Heidelberg.
The Molecular Basis of Plant Development. 1989. R. Goldberg (ed.), Alan R. Liss, New York.
CCSCGP, V. 6.
Gautheret, R.J. 1985. History of plant tissue and cell culture: a personal account, pp. 1–59 in CCSCGP, V. 2.
CCSCGP, V. 3.
Vasil, I.K. and Vasil, V. 1980. Clonal propagation. Int. Rev. Cytol. Supp. 11A: 145–173.
Tissue Culture as a Plant Production System for Horticultural Crops. 1986. R.H. Zimmerman, R.J. Griesbach, F.A. Hammerschlag, and R.H. Lawson (eds.), Martinus Nijhoff Publishers, Dordrecht.
Levin, R., Gaba, V., Tal, B., Hirsch, S., DeNola, D. and Vasil, I.K. 1988. Automated plant tissue culture for mass propagation. Bio/Technology 6: 1035–1040.
Kartha, K.K. 1984. Elimination of viruses, pp. 577–585 in CCSCGP, V. 1.
Wang, P.J. and Hu, C.Y. 1980. Regeneration of virus-free plants through in vitro culture, pp. 61–99 in Advances in Biochemical Engineering, Volume 18. Plant Cell Cultures II. A. Fiechter (ed.), Springer-Verlag, Heidelberg.
Collins, G.B. and Grosser, J.W. 1984. Culture of embryos, pp. 241–257 in CCSCGP, V. 1.
Raghavan, V. 1986. Variability through wide crosses and embryo rescue, pp. 613–633 in CCSCGP, V. 3.
Nitzsche, W. and Wenzel, G. 1977. Haploids in plant breeding. Z. Pflanzcht. Supp. 8: 1–101.
Haploids of Higher Plants in vitro. 1986. H. Hu and H. Yang (eds.), Springer-Verlag, Heidelberg.
D'Amato, F. 1985. Cytogenetics of plant cell and tissue cultures and their regenerants. CRC Crit. Rev. Pl. Sci. 3: 73–112.
Vasil, I.K. 1988. Progress in the regeneration and genetic manipulation of cereal crops. Bio/Technology 6: 397–402.
Sacristan, M.D. 1986. Isolation and characterization of mutant cell lines and plants: disease resistance, pp. 513–525 in CCSCGP, V. 3.
Chaleff, R.S. 1986. Isolation and characterization of mutant cell lines and plants: herbicide-resistant mutants, pp. 499–512 in CCSCGP, V. 3.
Bourgin, J.P. 1986. Isolation and characterization of mutant cell lines and plants: auxotrophs and other conditional lethal mutants, pp. 475–498 in CCSCGP, V. 3.
Misra, S. and Gedamu, L. 1989. Heavy metal tolerant transgenic Brassica napus L. and Nicotiana tabacum L. plants. Theoret. App. Genet. 78: 161–168.
Hibberd, K.A., Anderson, P.A. and Barker, M. Tryptophan overproducer mutants of cereal crops. United States Patent No. 4,581,847.
Larkin, P.J. and Scowcroft, W.R. 1981. Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theoret. Appl. Genet. 60: 197–214.
Evans, D.A., Sharp, W.R. and Medina-Filho, H.P. 1984. Somaclonal and gameto-clonal variation. Amer. J. Bot. 71: 759–774.
Swedlund, B. and Vasil, I.K. 1985. Cytogenetic characterization of embryogenic callus and regenerated plants of Pennisetum americanum L. Theoret. Appl. Genet. 69: 575–581.
Karp, A. and Bright, S.W.J. 1985. On the causes and origins of somaclonal variation. Oxford Surv. Pl. Molec. Cell Biol. 2: 199–234.
CCSCGP, V. 4.
CCSCGP, V. 5.
Plant and Animal Cells: Process Possibilities. 1987. C. Webb and F. Mavituna (eds.), Ellis Horwood, Chichester.
Gleba, Y.Y. and Sytnik, K.M. 1984. Protoplast Fusion: Genetic Engineering in Higher Plants. Springer-Verlag, Heidelberg.
Sjodin, C. and Glimelius, K. 1989. Brassica naponigra, a somatic hybrid resistant to Phoma lingam . Theoret. Appl. Genet. 77: 651–656.
Gibson, R.W., Jones, M.G.K. and Fish, N. 1988. Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid Solarium tuberosum and S. brevidens . Theoret. Appl. Genet. 76: 113–117.
Sacristan, M.D., Gerdemann-Knorck, M. and Schieder, O. 1989. Incorporation of hygromycin resistance in Brassica nigra and its transfer to B. napus through asymmetric protoplast fusion. Theoret. Appl. Genet. 78: 194–200.
Kyozuka, J., Kaneda, T. and Shimamoto, K. 1989. Production of cytoplasmic male sterile rice (Oryza sativa L.) by cell fusion. Bio/Technology 7: 1171–1174.
Izhar, S. and Zelcher, A. 1986. Protoplast fusion and generation of cybrids for transfer of cytoplasmic male sterility, pp. 589–599 in CCSCGP, V. 3.
Padgette, S.R., della-Cioppa, G., Shah, D.M., Fraley, R.T. and Kishore, G.M. 1989. Selective herbicide tolerance through protein engineering, pp. 441–476 in CCSCGP, V. 6.
Vaeck, M., Reynaerts, A. and Hofte, H. 1989. Protein engineering in plants: expression of Bacillus thuringiensis insecticidal protein genes, pp. 425–439 CCSCGP, V. 6.
Delannay, X., LaVallee, B.J., Proksch, R.K., Fuchs, R.L., Sims, S.R., Greenplate, J.T., Marrone, P.G., Dodson, R.B., Augustine, J.J., Layton, J.G. and Fischoff, D.A. 1989. Field performance of transgenic tomato plants expressing the Bacillus thuringiensis var. kurstaki insect control protein. Bio/Technology 7: 1265–1269.
Hemenway, C., Turner, N.E., Powell, P.A. and Beachy, R.N. 1989. Genetic engineering of plants for viral disease resistance, pp. 405–423 in CCSCGP, V. 6.
Stark, D.M. and Beachy, R.N. 1989. Protection against potyvirus infection in transgenic plants: evidence for broad spectrum resistance. Bio/Technology 7: 1257–1262.
Paszkowski, J., Baur, M., Bogucki, A. and Potrykus, I. 1988. Gene targeting in plants. EMBO J. 7: 4021–4026.
Kramer, M., Sheehy, R.E. and Hiatt, W.R. 1989. Progress towards the genetic engineering of tomato fruit softening. TIB-TECH 7: 191–194.
Innovative Approaches to Plant Disease Control. 1987. I. Chet (ed.), John Wiley, New York.
Timberlake, W.E. and Marshall, M.A. 1989. Genetic engineering of filamentous fungi. Science 244: 1313–1317.
Lindow, S.E., Panopoulos, N.J. and McFarland, B.L. 1989. Genetic engineering of bacteria from managed and natural habitats. Science 244: 1300–1306.
Gasser, C.S. and Fraley, R.T. 1989. Genetically engineered plants for crop improvement. Science 244: 1293–1299.
Vandekerckhove, J., Van Damme, J., van Lijsebettens, M., Botterman, J., de Block, M., Vandewiele, M., de Clercq, A., Lee-mans, J., van Montagu, M. and Krebbers, E. 1989. Enkephalins produced in transgenic plants using modified 2S seed storage proteins. Bio/Technology 7: 929–932.
Hiatt, A., Cafferkey, R. and Bowdish, K. 1989. Production of antibodies in transgenic plants. Nature 342: 76–78.
Duvick, D.N. 1989. Research collaboration and technology transfer: the public and private sectors in developing countries and the international seed companies, pp. 21–32 in Strengthening Collaboration in Biotechnology: International Agricultural Research and the Private Sector. J. I. Cohen (ed.), Agency for International Development, Washington, DC.
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Vasil, I. The Realities and Challenges of Plant Biotechnology. Nat Biotechnol 8, 296–301 (1990). https://doi.org/10.1038/nbt0490-296
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DOI: https://doi.org/10.1038/nbt0490-296
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