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Three-dimensional structure of the RGD-containing neurotoxin homologue dendroaspin

Nature Structural Biology volume 1, pages 802–807 (1994)Cite this article

Abstract

Dendroaspin is a short chain neurotoxin homologue from the venom of Elapidae snakes, which lacks neurotoxicity. Unlike neurotoxins, it contains an Arg-Gly-Asp-(RGD)-motif and functions as an inhibitor of platelet aggregation and platelet adhesion with comparable potency to the disintegrins from the venoms of Viperidae. We have determined the structure of dendroaspin in solution using NMR spectroscopy. The structure contains a core similar to that of short chain neurotoxins, but with a novel arrangement of loops and a solvent-exposed RGD-motif. Dendroaspin is thus an integrin antagonist with a well defined fold different from that of the disintegrins, based on the neurotoxin scaffold.

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Author notes

  1. Michael J. Sutcliffe

    Present address: Department of Chemistry, University of Leicester, Leicester, LE1 7RH, U.K.

Authors and Affiliations

  1. Biological NMR Centre, University of Leicester, P.O.Box 138, Leicester, LE1 9HN, U.K.

    Michael J. Sutcliffe

  2. School of Biochemistry, University of Birmingham, Edgbaston, Birmingham, B15 2TT, U.K.

    Mahesh Jaseja & Eva I. Hyde

  3. Thrombosis Research Institute, Emmanuel Kaye Building, Manresa Rd., London, SW3 6LR, U.K.

    Xinjie Lu

  4. Department of Applied Pharmacology, National Heart and Lung Institute, Dovehouse Street, London, SW3 6LY, U.K.

    Janice A. Williams

Authors
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  4. Xinjie Lu
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  5. Janice A. Williams
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Sutcliffe, M., Jaseja, M., Hyde, E. et al. Three-dimensional structure of the RGD-containing neurotoxin homologue dendroaspin. Nat Struct Mol Biol 1, 802–807 (1994). https://doi.org/10.1038/nsb1194-802

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